1. Uhlolojikelele

Bheka izibalo ezilandelayo ngenkathi ufunda lesi sahluko.

1.1 Isingeniso Esiyinhloko

1.1.1 Amazinga

I-ISO/DIS 22611 Izingubo zokuvikela kuma-ejenti athathelwanayo-Indlela yokuhlola ukumelana nokungena ngama-aerosol angcoliswe yibhayoloji.

1.1.2 Imininingwane

lIjeneretha ye-aerosol:    I-Atomizer

lIgumbi lokuvezwa:  PMMA

lUkuhlanganiswa kwesampula :2, insimbi engagqwali

lIphampu ye-vacum:Kufika ku-80kpa

lUbukhulu: 300mm*300mm*300mm

lUkunikezwa kwamandla kagesi  :220V 50-60Hz

l Ubukhulu bomshini: 46cm×93cm×49cm (H)

l Isisindo Esiphelele: 35kg

2. UKUSETSHENZISWA KWESIZINSI

2.1 Ukulungiselela

Faka izingxenye ezintathu kukhabhinethi ye-biosafety. Hlola ingxenye ngayinye yomshini wokuhlola futhi uqiniseke ukuthi zonke izingxenye zisebenza kahle futhi zixhuma kahle.

Ukusika amasampula ayisishiyagalombili njengendilinga engu-25mm ububanzi.

Lungiselela isiko lasebusuku le-Staphylococcus aureus ngokudluliswa kwe-aseptic kwebhaktheriya isuka ku-agar yezakhi (egcinwe ku-4±1℃) iyiswe kumhluzi wezakhi kanye ne-incubation ku-37±1℃ ku-shaker ye-orbital.

Nciphisa isiko libe umthamo ofanele we-isotonic saline oyinyumba ukuze unikeze inani lokugcina lebhaktheriya elilinganiselwa ku-5*10.⁷amaseli cm^-3usebenzisa igumbi lokubala amagciwane e-Thoma.

Gcwalisa isiko elingenhla ku-atomizer. Izinga loketshezi liphakathi kwezinga eliphezulu neliphansi.

2.2 Ukusebenza

Faka umhlangano wesampula. Faka i-silicone washer A, indwangu yokuhlola, iwasha ye-silicone B, ulwelwesi, ukusekela ngocingo esivaloni esivulekile, ikhava ngesisekelo.

Faka enye isampula yokuhlanganisa ngaphandle kwesampula.

Vula isivalo esingaphezulu segumbi lokuhlola.

Faka umhlangano wesampula ngesampula kanye nomhlangano ngaphandle kwesampula nge-Fasten of Fig. 4-1.

Qinisekisa ukuthi wonke amashubhu axhumeke kahle.

Xhuma umoya ocindezelwe ukuze ulungise umoya ocindezelweyo.

Faka umoya ekugelezeni kuka-5L/min ngokulungisa imitha yokugeleza ku-atomizer bese uqala ukukhiqiza i-aerosol.

Ngemuva kwemizuzu emi-3 vula iphampu ye-vacumm. Ibeke ibe ngu-70kpa.

Ngemuva kwemizuzu engu-3, ​​vala umoya ku-atomizer, kodwa shiya iphampu ye-vacuum isebenza iminithi elingu-1.

Vala iphampu yokugomela.

Susa amasampula amasampula ekamelweni. Futhi dlulisa ulwelwesi lwe-0.45um ngokungenasisekelo emabhodleleni asemhlabeni wonke aqukethe u-10ml oyinyumba we-isotonic saline.

Khipha ngokunyakazisa iminithi elingu-1. Futhi wenze ukuhlanjululwa kwe-serial nge-saline oyinyumba. (10^-1, 10^-2, 10^-3, futhi 10^-4)

Faka ama-aliquots angu-1ml we-dilution ngayinye ngokuphindwe kabili usebenzisa i-agar enomsoco.

Gcoba amapuleti ngobusuku obungu-37±1℃ futhi uveze imiphumela usebenzisa isilinganiso sesibalo sebhaktheriya sangemuva nenani le-bacterium elidlule ngesampula yokuhlola.

Yenza izinqumo ezine ngohlobo lwendwangu ngayinye noma isimo sendwangu.

3. UKONNDWA

Njengazo zonke izinto ezisebenza ngogesi, le yunithi kufanele isetshenziswe ngendlela efanele futhi ukunakekelwa nokuhlolwa kufanele kwenziwe njalo ngezikhathi ezithile. Izinyathelo ezinjalo zokuqapha zizoqinisekisa ukusebenza okuphephile nokusebenza kahle kwemishini.

Ukunakekela ngezikhathi ezithile kuhlanganisa ukuhlolwa okwenziwa ngokuqondile ngumqhubi wokuhlola kanye/noma izisebenzi zesevisi ezigunyaziwe.

Ukunakekela okokusebenza kuwumthwalo womthengi futhi kufanele kwenziwe njengoba kushiwo kulesi sahluko.

Ukwehluleka ukwenza izenzo zokulungisa ezinconyiwe noma ukunakekela okwenziwa abantu abangagunyaziwe kungachitha iwaranti.

1. Umshini kufanele uhlolwe ukuze uvimbele ukuvuza kokuxhumeka ngaphambi kokuhlolwa;

2. Ukuhambisa umshini akuvunyelwe uma uwusebenzisa;

3. Khetha amandla ahambisanayo kanye ne-voltage. Ungabi phezulu kakhulu ukugwema ukushisa idivayisi;

4. Sicela usithinte ukuze uphathe ngesikhathi lapho umshini ungasebenzi;

5. Kufanele ibe nendawo enhle yokukhipha umoya lapho umshini usebenza;

6. Ukuhlanza umshini ngemva kokuhlolwa njalo;