Amapharamitha wethuluzi le-PCR le-Touch Fluorescence quantitative

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Inombolo yemodeli: CFX96 Touch 1. Indawo yokusebenza 1.1 Izinga lokushisa elisebenzayo 5-31℃ 1.2 Umswakama Osebenzayo Umswakama ohlobene ≤80% 1.3 Ukunikezwa kwamandla okusebenzayo 100-240 VAC, 50-60Hz. 2. Umsebenzi Ingasetshenziswa ku-nucleic acid quantification, ukuhlaziywa kwezinga lesisho sofuzo, ukutholwa kokuguqulwa kofuzo, ukutholwa kwe-GMO kanye nokuhlaziywa kwemininingwane yomkhiqizo kanye neminye imikhakha yocwaningo. 3. Izimfuneko zokusebenza nezobuchwepheshe 3.1 Ukusebenza Okuyinhloko (* inkomba okufanele kuhlangatshezwane nayo) *3.1.1 Ishaneli etholwayo eyisithupha...


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    Omaka bomkhiqizo

    Inombolo yemodeli: CFX96 Touch


    1. Indawo yokusebenza

    1.1 Izinga lokushisa lokusebenza 5-31℃

    1.2 Umswakamo Osebenzayo Umswakama ohlobene ≤80%

    1.3 Amandla asebenzayo 100-240 VAC, 50-60Hz.

     

    2. Fukuqaqa

    Ingasetshenziswa ku-nucleic acid quantification, ukuhlaziywa kwezinga lesisho sofuzo, ukutholwa kokuguqulwa kofuzo,

    Ukutholwa kwe-GMO nokuhlaziywa kwemininingwane yomkhiqizo kanye neminye imikhakha yocwaningo.

    3. Izidingo zokusebenza kanye nezobuchwepheshe

    3.1 Ukusebenza Okubalulekile (* inkomba okufanele kuhlangatshezwane nayo)

    *3.1.1 Iziteshi zokuthola eziyisithupha, ezikwazi ukubona i-PCR ephindwe ka-5, zingathola ngasikhathi sinye izakhi zofuzo eziqondiwe ezi-5, kanye nesiteshi esikhethekile sokuthola i-FRET

    *3.1.2 Ngokusebenza kwe-PCR yezinga lokushisa eliguquguqukayo, amazinga okushisa angu-8 ahlukene angaqhutshwa ngesikhathi esisodwa, futhi isikhathi sokufukamela sezinga lokushisa ngalinye siyefana

    3.1.3 I-reagent evuleke ngokuphelele, elungele zonke izinhlobo zocwaningo lwesayensi kanye nezinto ezisebenza ngomtholampilo

    3.1.4 Ifanele izindlela ezehlukene ze-fluorescence, njenge-Taqman, i-Molecular Beacon, i-FRET probe, i-SYBR Green I, njll.

    3.1.5 Izinto ezisetshenziswayo zivuliwe futhi zingasebenzisa ipayipi elilodwa elingu-0.2ml, ipayipi eliyisishiyagalombili, ipuleti lemithombo engu-96, njll.

    *3.1.6 Ingasebenza ngokuzimela futhi isebenze ingaxhunyiwe ku-inthanethi. Ijika le-PCR fluorescence amplification yesikhathi sangempela ingagadwa ngaphandle koxhumano lwekhompyutha

     

    3.2 Izidingo zobuchwepheshe eziyinhloko (* inkomba okufanele ihlangatshezwe)

    *3.2.1 Umthamo wesampula: 96×0.2ml, ungasetshenziswa ukucaciswa okujwayelekile okungu-96-well plate (12×8)

    Uhlobo lwezinto ezisetshenziswayo: 0.2ml ipayipi elilodwa, ipayipi eliyisishiyagalombili, ipuleti lemithombo engu-96, njll

    3.2.3 Isistimu yokusabela: 1-50µ L (10-25µ L iyanconywa)

    *3.2.4 Umthombo wokukhanya: amaledi ayisithupha anezihlungi

    *3.2.5 Umtshina: ama-diode ayisithupha e-photosensitive anezihlungi

    *3.2.6 izinga lokushisa lokwehla: 5℃/ s

    3.2.7 Ibanga lokulawula izinga lokushisa: 0-100 ℃

    Ukunemba kwezinga lokushisa: ±0.2℃ (ku-90˚C)

    Ukufana kwezinga lokushisa: ±0.4℃ (kufika ku-90˚C phakathi kwamasekhondi angu-10)

    *3.2.10 Umsebenzi we-gradient wokushisa oguqukayo: sebenzisa amazinga okushisa angu-8 ahlukene ngesikhathi esisodwa; Ibanga lokulawula izinga lokushisa le-Gradient: 30-100 ℃; Ibanga lokushisa le-gradient: 1-24 ℃; Isikhathi sokufukamela ezingeni lokushisa le-gradient: okufanayo

    3.2.11 Ibanga lobude be-wavelength obujabulisayo/okukhiphayo: 450-730nm

    3.2.12 Ukuzwela: Ingakwazi ukubona ikhophi eyodwa yofuzo ku-genome yomuntu

    3.2.13 Ibanga elinamandla: ama-oda ayi-10 obukhulu

    3.2.14 Isibonisi: 8.5-intshi umbala touch screen

    3.2.15 Imodi yokuhlaziya idatha: ijika elivamile lobuningi, ijika elihlanganisiwe, ukuhlaziywa kwe-CT noma δ δ CT CT, ukuhlaziywa kwezakhi zofuzo eziningi zereferensi nokubalwa kokusebenza kahle kokukhulisa, ukuhlaziya isisho sofuzo samafayela edatha eminingi, ukuhlaziywa kwe-allele, ukuhlaziya iphuzu lokugcina, nama-alleles. , ukuhlaziya ijika le-fusion

    3.2.16 Ukuthunyelwa Kwedatha: Excel, Word, noma PowerPoint. Imibiko yabasebenzisi iqukethe qalisa Izilungiselelo, imiphumela yedatha enemifanekiso nethebula engaphrintwa ngokuqondile noma ilondolozwe njenge-PDF.

    *3.2.17 Ucwaningo lwesakhiwo se-Chromosome: Indlela ye-PCR yesikhathi sangempela yasetshenziswa ukuze kuhlaziywe ngokwesilinganiso ukwakheka kwe-chromatin ngokuqhathanisa imiphumela yama-nuclease ekonakaleni kwe-genomic DNA. Lokhu kukhombisa ngempela ukuhlobana okuphezulu phakathi kwesakhiwo se-chromatin nesisho sofuzo

    4 Izesekeli Ezidingekayo

    Isofthiwe yokuhlaziya ikhompuyutha nokulawula (okuhlanganisa ukulinganisa okuphelele, ukulinganisa okuhlobene, ukuhlaziya ijika elincibilikayo, ukuhlaziya amaphuzu wokugcina, ukuqhathanisa idatha yamabhodi amaningi, njll.)

     

    5 Isikhathi sokuqinisekisa ikhwalithi

    Isikhathi sesiqinisekiso sekhwalithi sizoba unyaka owodwa ngemuva kokuthi ukufakwa nokusetshenziswa kwamukelwe futhi kwamukelwe umsebenzisi.


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