Touch Fluorescence quantitative PCR isixhobo parameters

Inombolo yomzekelo: CFX96 Touch

1. Indawo yokusebenza

1.1 Ubushushu bokusebenza 5-31℃

1.2 Ukufuma okuSebenzayo Ukufuma okunxulumeneyo ≤80%

1.3 Ukusebenza kwamandla okusebenza 100-240 VAC, 50-60Hz.

2. Fukukhulula

Ingasetyenziswa kwi-nucleic acid quantification, uhlalutyo lwenqanaba lokuchazwa kofuzo, ukufumanisa ukuguqulwa kofuzo,

Ukufunyanwa kwe-GMO kunye nohlalutyo oluthile lwemveliso kunye nezinye iinkalo zophando.

3. Iimfuno zokusebenza kunye nezobugcisa

3.1 UMsebenzi oPhambili (* sisalathi ekufuneka sihlangatyezwe)

* 3.1.1 Imijelo emithandathu yokufumanisa, enokuqonda i-PCR ye-5-fold, inokubona ngaxeshanye i-5 yejene ekujoliswe kuyo, kunye nesiteshi esikhethekileyo sokufumanisa i-FRET

*3.1.2 Ngomsebenzi wePCR weqondo lobushushu eliguquguqukayo, amaqondo obushushu ahlukeneyo ayi-8 anokuqhutywa ngaxeshanye, kwaye ixesha lokufukamela lobushushu ngalinye liyafana.

3.1.3 Ireyinti evulwe ngokupheleleyo, ilungele zonke iintlobo zophando lwenzululwazi kunye neerejenti zeklinikhi

3.1.4 Ifanelekile kwiindlela ezahlukeneyo ze-fluorescence, ezifana ne-Taqman, i-Molecular Beacon, i-FRET probe, i-SYBR Green I, njl.

3.1.5 Izinto ezisetyenziswayo zivulekile kwaye zingasebenzisa i-0.2ml umbhobho omnye, umbhobho osibhozo, ipleyiti ye-96, njl.

*3.1.6 Ingakwazi ukuzimela kwaye isebenze ngaphandle kweintanethi. Ixesha lokwenyani i-PCR fluorescence amplification curve inokubekwa iliso ngaphandle koqhagamshelo lwekhompyuter

3.2 Iimfuno eziphambili zobugcisa (* sisalathi ekufuneka sifezekiswe)

* 3.2.1 Umthamo wesampuli: 96 × 0.2ml, ingasetyenziswa inkcazo esemgangathweni ye-96-well plate (12 × 8)

Uhlobo lwezinto ezisetyenziswayo: i-0.2ml ipayipi enye, ibhobho esibhozo, i-96-well plate, njl

3.2.3 Inkqubo yokusabela: 1-50µ L (10-25µ L iyacetyiswa)

*3.2.4 Umthombo wokukhanya: iiledi ezintandathu ezinezihluzi

*3.2.5 Umtshina: iidiode ezintandathu ezinezihluzi

*3.2.6 izinga lobushushu lokuhla: 5℃/ s

3.2.7 Uluhlu lokulawula ubushushu: 0-100 ℃

Ukuchaneka kobushushu: ±0.2℃ (ku-90˚C)

Ubushushu obufanayo: ±0.4℃ (ukuya kuthi ga kwi-90˚C phakathi kwemizuzwana eyi-10)

*3.2.10 Umsebenzi weqondo lobushushu obunamandla: sebenzisa amaqondo obushushu ahlukeneyo ayi-8 ngaxeshanye; Uluhlu lolawulo lweqondo lobushushu: 30-100 ℃; Uluhlu lweqondo lokushisa: 1-24 ℃; Ixesha lokufukamela kwiqondo lobushushu legradient: kuyafana

3.2.11 Uchulumanco/ukukhutshwa koluhlu lwamaza: 450-730nm

3.2.12 Uvakalelo: Iyakwazi ukubona ikopi enye yemfuza kwigenome yomntu

3.2.13 Uluhlu olutshintshayo: Iiodolo ezili-10 zobukhulu

3.2.14 Bonisa: 8.5-intshi umbala touch screen

3.2.15 Imowudi yohlalutyo lwedatha: ijika lomgangatho wobungakanani, igophe elidityanisiweyo, iCT okanye δ δ CT uhlalutyo lokuchazwa kofuzo, uhlalutyo lwangaphakathi lwereferensi yereferensi kunye nokubalwa kobuchule bokukhulisa, uhlalutyo lokuchazwa kofuzo lweefayile ezininzi zedatha, uhlalutyo lwe-allele, uhlalutyo lwamanqaku okuphela, kunye ne-alleles. , uhlalutyo lwegophe lokudibanisa

3.2.16 Ukuthunyelwa ngaphandle kwedatha: Excel, Word, okanye PowerPoint. Iingxelo zabasebenzisi ziqulathe useto olusebenzayo, iziphumo zegraphical kunye netheyibhile ezinokuprintwa ngokuthe ngqo okanye zigcinwe njengePDF.

* I-3.2.17 Isifundo sesakhiwo se-Chromosome: Indlela ye-PCR yexesha langempela isetyenziswe ukuhlalutya ubungakanani besakhiwo se-chromatin ngokuthelekisa iziphumo ze-nuclease kwi-genomic DNA degradation. Oku kubonisa ngenene unxulumano oluphezulu phakathi kolwakhiwo lwechromatin kunye nokubonakaliswa kofuzo

4 Izinto eziyimfuneko

Isoftware yohlalutyo lwekhompyuter kunye nolawulo (kubandakanya ubungakanani obupheleleyo, ubungakanani obuhambelanayo, uhlalutyo olunyibilikayo lwegophe, uhlalutyo lwesiphelo, uthelekiso lwedatha yeebhodi ezininzi, njl.njl.)

5 Ixesha leSiqinisekiso soMgangatho

Ixesha lokuqinisekisa umgangatho liya kuba ngunyaka omnye emva kokufakela kunye nokugunyaziswa kwamkelwe kwaye kwamkelwe ngumsebenzisi.

SHANDONG DRICK Instruments CO.,LTD

Iprofayile yekhampani

I-Shandong Drick Instruments Co., Ltd, ibandakanyeka ikakhulu kuphando kunye nophuhliso, ukuveliswa kunye nokuthengiswa kwezixhobo zokuvavanya.

Inkampani yasekwa ngo-2004.

Iimveliso zisetyenziswa kwiiyunithi zophando lwezenzululwazi, amaziko okuhlola umgangatho, iiyunivesithi, ukupakishwa, iphepha, ushicilelo, irabha kunye neeplastiki, iikhemikhali, ukutya, amayeza, amalaphu, kunye namanye amashishini.
UDrick unika ingqwalasela ekulimeni italente kunye nokwakhiwa kweqela, ukubambelela kumbono wophuhliso wobuchule, ukuzinikela.pragmatism, kunye nokusungula izinto ezintsha.
Ukuthobela umgaqo ojolise kubathengi, ukusombulula ezona mfuno zingxamisekileyo nezisebenzayo zabathengi, kwaye unikeze izisombululo zeklasi yokuqala kubathengi abaneemveliso ezikumgangatho ophezulu kunye nobuchwepheshe obuphambili.