Touch Fluorescence quantitative PCR isixhobo parameters

Inombolo yomzekelo: CFX96 Touch

1. Indawo yokusebenza

1.1 Ubushushu bokusebenza 5-31℃

1.2 Ukufuma okuSebenzayo Ukufuma okunxulumeneyo ≤80%

1.3 Ukusebenza kwamandla okusebenza 100-240 VAC, 50-60Hz.

2. Fukukhulula

Ingasetyenziswa kwi-nucleic acid quantification, uhlalutyo lwenqanaba lokuchazwa kofuzo, ukufumanisa ukuguqulwa kofuzo,

Ukufunyanwa kwe-GMO kunye nohlalutyo oluthile lwemveliso kunye nezinye iinkalo zophando.

3. Iimfuno zokusebenza kunye nezobugcisa

3.1 UMsebenzi oPhambili (* sisalathi ekufuneka sihlangatyezwe)

* 3.1.1 Imijelo emithandathu yokufumanisa, enokuqonda i-PCR ye-5-fold, inokubona ngaxeshanye i-5 yejene ekujoliswe kuyo, kunye nesiteshi esikhethekileyo sokufumanisa i-FRET

*3.1.2 Ngomsebenzi wePCR weqondo lobushushu eliguquguqukayo, amaqondo obushushu ahlukeneyo ayi-8 anokuqhutywa ngaxeshanye, kwaye ixesha lokufukamela lobushushu ngalinye liyafana.

3.1.3 Ireyinti evulwe ngokupheleleyo, ilungele zonke iintlobo zophando lwenzululwazi kunye neerejenti zeklinikhi

3.1.4 Ifanelekile kwiindlela ezahlukeneyo ze-fluorescence, ezifana ne-Taqman, i-Molecular Beacon, i-FRET probe, i-SYBR Green I, njl.

3.1.5 Izinto ezisetyenziswayo zivulekile kwaye zingasebenzisa i-0.2ml umbhobho omnye, umbhobho osibhozo, ipleyiti ye-96, njl.

*3.1.6 Ingakwazi ukuzimela kwaye isebenze ngaphandle kweintanethi. Ixesha lokwenyani i-PCR fluorescence amplification curve inokubekwa iliso ngaphandle koqhagamshelo lwekhompyuter

3.2 Iimfuno eziphambili zobugcisa (* sisalathi ekufuneka sifezekiswe)

* 3.2.1 Umthamo wesampuli: 96 × 0.2ml, ingasetyenziswa inkcazo esemgangathweni ye-96-well plate (12 × 8)

Uhlobo lwezinto ezisetyenziswayo: i-0.2ml ipayipi enye, ibhobho esibhozo, i-96-well plate, njl

3.2.3 Inkqubo yokusabela: 1-50µ L (10-25µ L iyacetyiswa)

*3.2.4 Umthombo wokukhanya: iiledi ezintandathu ezinezihluzi

*3.2.5 Umtshina: iidiode ezintandathu ezinezihluzi

*3.2.6 izinga lobushushu lokuhla: 5℃/ s

3.2.7 Uluhlu lokulawula ubushushu: 0-100 ℃

Ukuchaneka kobushushu: ±0.2℃ (ku-90˚C)

Ubushushu obufanayo: ±0.4℃ (ukuya kuthi ga kwi-90˚C phakathi kwemizuzwana eyi-10)

*3.2.10 Umsebenzi weqondo lobushushu obunamandla: sebenzisa amaqondo obushushu ahlukeneyo ayi-8 ngaxeshanye; Uluhlu lolawulo lweqondo lobushushu: 30-100 ℃; Uluhlu lweqondo lokushisa: 1-24 ℃; Ixesha lokufukamela kwiqondo lobushushu legradient: kuyafana

3.2.11 Uchulumanco/ukukhutshwa koluhlu lwamaza: 450-730nm

3.2.12 Uvakalelo: Iyakwazi ukubona ikopi enye yemfuza kwigenome yomntu

3.2.13 Uluhlu olutshintshayo: Iiodolo ezili-10 zobukhulu

3.2.14 Bonisa: 8.5-intshi umbala touch screen

3.2.15 Imowudi yohlalutyo lwedatha: ijika lomgangatho wobungakanani, igophe elidityanisiweyo, iCT okanye δ δ CT uhlalutyo lokuchazwa kofuzo, uhlalutyo lwangaphakathi lwereferensi yereferensi kunye nokubalwa kobuchule bokukhulisa, uhlalutyo lokuchazwa kofuzo lweefayile ezininzi zedatha, uhlalutyo lwe-allele, uhlalutyo lwamanqaku okuphela, kunye ne-alleles. , uhlalutyo lwegophe lokudibanisa

3.2.16 Ukuthunyelwa ngaphandle kwedatha: Excel, Word, okanye PowerPoint. Iingxelo zabasebenzisi ziqulathe useto olusebenzayo, iziphumo zegraphical kunye netheyibhile ezinokuprintwa ngokuthe ngqo okanye zigcinwe njengePDF.

* I-3.2.17 Isifundo sesakhiwo se-Chromosome: Indlela ye-PCR yexesha langempela isetyenziswe ukuhlalutya ubungakanani besakhiwo se-chromatin ngokuthelekisa iziphumo ze-nuclease kwi-genomic DNA degradation. Oku kubonisa ngenene unxulumano oluphezulu phakathi kolwakhiwo lwechromatin kunye nokubonakaliswa kofuzo

4 Izinto eziyimfuneko

Isoftware yohlalutyo lwekhompyuter kunye nolawulo (kubandakanya ubungakanani obupheleleyo, ubungakanani obuhambelanayo, uhlalutyo olunyibilikayo lwegophe, uhlalutyo lwesiphelo, uthelekiso lwedatha yeebhodi ezininzi, njl.njl.)

5 Ixesha leSiqinisekiso soMgangatho

Ixesha lokuqinisekisa umgangatho liya kuba ngunyaka omnye emva kokufakela kunye nokugunyaziswa kwamkelwe kwaye kwamkelwe ngumsebenzisi.