Umgaqo wokusebenza weKjeldahl nitrogen ukuzimisela

Ngokomgaqo we-Kjeldahl nitrogen determination, amanyathelo amathathu afunekayo ukuze kumiselwe, oko kukuthi, ukugaya, ukuchithwa kunye ne-titration.

Ukwetyisa: Fudumeza initrogen equlathe iikhompawundi eziphilayo (iiprotheyini) kunye ne-asidi yesulfuric exutyiweyo kunye neecatalysts (icopper sulfate okanye iKjeldahl digestion tablets) ukubolisa iproteni. Ikhabhoni kunye ne-hydrogen zifakwa kwi-carbon dioxide kunye namanzi ukubaleka, ngelixa i-nitrogen ye-organic iguqulwa ibe yi-ammonia (NH3) kwaye idibaniswe ne-asidi ye-sulfuric ukwenza i-ammonium sulfate. (I-Ammonium NH4+)

Inkqubo yokugaya: ukufudumeza ngobushushu obuphantsi ukubilisa, i-substance kwi-flask i-carbonized kwaye imnyama, kwaye ubuninzi be-foam iveliswa. Emva kokuba i-foam ilahlekile, yongeza i-firepower ukugcina imeko yokubilisa kancinane. Xa ulwelo luba luhlaza okwesibhakabhaka kwaye lucacile, qhubeka ushushu kwi-05-1h, kwaye upholile emva kokuphela. (Ungasebenzisa isixhobo sokwetyisa esizenzekelayo ukugqiba umsebenzi wokulungiswa kwangaphambili)

I-Distillation: Isisombululo esifunyenweyo sihlanjululwa kwivolumu eqhubekayo kwaye yongezwa kunye ne-NaOH ukukhulula i-NH3 nge-distillation. Emva kwe-condensation, iqokelelwa kwisisombululo se-boric acid.

Inkqubo ye-distillation: Okokuqala, isampuli egayiweyo ihlanjululwe, i-NaOH yongezwa, kunye negesi ye-ammonia eyenziwa emva kokufudumeza ingena kwi-condenser, kwaye igeleza kwibhotile efumana isisombululo se-boric acid emva kokuxutywa. Yenza i-ammonium borate. (Isalathisi esixubileyo songezwa kwisisombululo se-boric acid. Emva kokuba i-ammonium borate yenziwe, isisombululo sokufunxa sitshintsha ukusuka kwi-acidic ukuya kwi-alkaline, kwaye umbala uguquka ukusuka kwimfusa ukuya eluhlaza okwesibhakabhaka.)

I-Titration: I-Titrate kunye nesisombululo esiqhelekileyo se-hydrochloric acid ye-concentration eyaziwayo, bala umxholo we-nitrogen ngokwexabiso le-hydrochloric acid esetyenzisiweyo, kwaye uyiphindaphinde nge-conversion factor ehambelanayo ukufumana umxholo weprotheni. (I-Titration ibhekisa kwindlela yokuhlalutya ubungakanani kunye nokusebenza kovavanyo lwekhemikhali. Isebenzisa ukusabela kobungakanani bezisombululo ezibini ukugqiba umxholo wesolute ethile. Ibonisa indawo yokugqibela yetitration ngokokutshintsha kombala wesalathisi, kwaye emva koko ujonge ukusetyenziswa kwesisombululo esisemgangathweni Umthamo, izibalo kunye neziphumo zohlalutyo.)

Inkqubo ye-Titration: Yehlisa isisombululo esisemgangathweni se-hydrochloric acid kwisisombululo se-ammonium borate ukutshintsha umbala wesisombululo ukusuka eluhlaza okwesibhakabhaka ukuya kubomvu okhanyayo.

DRK-K616 oluzenzekelayo Kjeldahl nitrogen analyzersisihlalutyi esikrelekrele esizenzekelayo sokumisela umxholo wenitrogen esekwe kwindlela yeKjeldahl. Ingasetyenziswa ngokubanzi kulungiso lokutya, imveliso yokutya, icuba, ukufuywa kwezilwanyana, isichumiso somhlaba, ukubeka iliso kokusingqongileyo, amayeza, ezolimo, uphando lwezenzululwazi, ukufundisa, ukubeka iliso kubulunga kunye nezinye iinkalo zohlalutyo lwenitrogen kunye neprotheyini kwi-macro kunye ne-semi-micro. iisampulu. Ingasetyenziselwa ityuwa ye-ammonium, Ukufunyanwa kwe-fatty acids / alkali eguqukayo, njl. I-distillation kunye ne-titration yizona nkqubo zokulinganisa eziphambili ze-DRK-K616 Kjeldahl nitrogen analyzer. I-DRK-K616 uhlobo lwe-Kjeldahl nitrogen analyzer yinkqubo yokulinganisa i-nitrogen ezenzekelayo ngokupheleleyo kunye ne-titration ye-nitrogen eyilwe ngokwendlela yokugqiba i-Kjeldahl nitrogen; esi sixhobo sibonelela ngokulula kakhulu kubavavanyi belabhoratri kwinkqubo yokumisela i-nitrogen-protein. , Kwaye uneempawu zokusetyenziswa okukhuselekileyo nokuthembekileyo; umsebenzi olula kunye nokugcina ixesha. I-interface ye-dialogue yaseTshayina yenza umsebenzisi asebenze lula, i-interface inobubele, kwaye ulwazi olubonisiweyo lutyebile, ukwenzela ukuba umsebenzisi angakwazi ukuqonda ngokukhawuleza ukusetyenziswa kwesixhobo.

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