Touch Fluorescence quantitative PCR lisebelisoa tsa paramente

Nomoro ea mohlala: CFX96 Touch

1. Tikoloho ea mosebetsi

1.1 Mocheso oa ho sebetsa 5-31℃

1.2 Mongobo o sebetsang Mongobo o lekanang ≤80%

1.3 Matla a sebetsang 100-240 VAC, 50-60Hz.

2. Fho qhaqhoa

E ka sebelisoa ho quantification ea nucleic acid, tlhahlobo ea boemo ba liphatsa tsa lefutso, ho lemoha phetoho ea liphatsa tsa lefutso,

Tlhahlobo ea GMO le tlhahlobo e ikhethang ea sehlahisoa le likarolo tse ling tsa lipatlisiso.

3. Litlhoko tsa ts'ebetso le tekheniki

3.1 Ts'ebetso e ka Sehloohong (* ke letšoao le lokelang ho finyelloa)

* 3.1.1 Mekhoa e tšeletseng ea ho lemoha, e ka khonang ho lemoha 5-fold PCR, ka nako e le 'ngoe e ka lemoha liphatsa tsa lefutso tsa 5, le mocha o khethehileng oa ho lemoha FRET

*3.1.2 Ka ts'ebetso ea PCR ea mocheso o matla, lithempereichara tse 8 tse fapaneng li ka tsamaisoa ka nako e le 'ngoe,' me nako ea ho incubation ea mocheso o mong le o mong oa tšoana.

3.1.3 Reagent e bulehileng ka botlalo, e loketseng mefuta eohle ea lipatlisiso tsa mahlale le lisebelisoa tsa bongaka

3.1.4 E loketse mekhoa e fapaneng ea fluorescence, joalo ka Taqman, Molecular Beacon, FRET probe, SYBR Green I, joalo-joalo.

3.1.5 Lisebelisoa li bulehile 'me li ka sebelisa phala e le' ngoe ea 0.2ml, phala e robeli, poleiti ea seliba sa 96, joalo-joalo.

*3.1.6 E ka sebetsa e ikemetse le ho sebetsa ntle le marang-rang. Nako ea 'nete ea PCR fluorescence amplification curve e ka shejoa ntle le khokahanyo ea khomphutha

3.2 Litlhoko tsa mantlha tsa tekheniki (* ke letšoao le lokelang ho finyelloa)

* 3.2.1 Sample capacity: 96×0.2ml, e ka sebelisoa maemo a tloaelehileng a 96-well plate (12×8)

Mofuta oa lisebelisoa: 0.2ml phala e le 'ngoe, phala e robeli, poleiti ea seliba sa 96, joalo-joalo

3.2.3 Sistimi ea ho arabela: 1-50µ L (10-25µ L e khothalelitsoe)

*3.2.4 Mohloli oa leseli: li-leds tse tšeletseng tse nang le li-filters

* 3.2.5 Detector: li-diode tse tšeletseng tsa lifoto tse nang le li-filters

*3.2.6 mocheso o theoha sekhahla: 5℃/ s

3.2.7 Lebelo la taolo ea mocheso: 0-100 ℃

Ho nepahala ha mocheso: ±0.2℃ (ho 90˚C)

Ho lumellana ha mocheso: ±0.4℃ (ho fihla ho 90˚C nakong ea metsotsoana e 10)

*3.2.10 Ts'ebetso ea matla a matla a mocheso: tsamaisa mocheso o fapaneng oa 8 ka nako e le 'ngoe; Lebelo la taolo ea mocheso oa likhahla: 30-100 ℃; Mocheso oa mocheso oa likhahla: 1-24 ℃; Nako ea ho qhoqhoa ka mocheso oa gradient: ho tšoana

3.2.11 Phatlalatso / wavelength ea mosi: 450-730nm

3.2.12 Sensitivity: E khona ho lemoha kopi e le 'ngoe ea lefutso ho genome ea motho

3.2.13 Dynamic range: 10 orders of magnitude

3.2.14 Pontšo: 8.5-inch color touch screen

3.2.15 Mokhoa oa ho hlahloba lintlha: quantitative standard curve, fusion curve, CT kapa δ δ CT gene expression analysis, multiple internal reference gene analysis and amplification performance calculation, gene expression of multiple data files, allele analysis, end point analysis, with alleles. , fusion curve analysis

3.2.16 Thomello ya Boitsebiso: Excel, Word, kapa PowerPoint. Litlaleho tsa mosebelisi li na le Litlhophiso tse tsamaeang, liphetho tsa data tse hlakileng le tse thathamisitsoeng tse ka hatisoang ka kotloloho kapa tsa bolokoa joalo ka PDF.

*3.2.17 Thuto ea sebopeho sa Chromosome: Mokhoa oa sebele oa PCR o ne o sebelisetsoa ho hlahloba ka mokhoa o leka-lekaneng sebopeho sa chromatin ka ho bapisa liphello tsa li-nuclease ho senyeha ha DNA ea genomic. Sena se hlile se bonts'a kamano e phahameng lipakeng tsa sebopeho sa chromatin le polelo ea lefutso

4 Lisebelisoa tse Hlokahalang

Software ea tlhahlobo ea khomphutha le taolo (ho kenyeletsoa palo e felletseng, palo e lekanyelitsoeng, tlhahlobo ea curve e qhibilihang, tlhahlobo ea lintlha, papiso ea lintlha tse ngata, jj.)

5 Quality Guarantee nako

Nako ea tiiso ea boleng e tla ba selemo se le seng ka mor'a hore ho kenngoa le ho romelloa ho amoheloe le ho amoheloa ke mosebedisi.

SHANDONG DRICK INSTRUMENTS CO.,LTD

Boemo ba Khoebo

Shandong Drick Instruments Co., Ltd, e sebetsa haholo ho etsa lipatlisiso le nts'etsopele, tlhahiso le thekiso ea lisebelisoa tsa tlhahlobo.

Khamphani e thehiloe ka 2004.

Lihlahisoa li sebelisoa likarolong tsa lipatlisiso tsa saense, litsi tsa tlhahlobo ea boleng, liunivesithi, liphutheloana, pampiri, khatiso, rabara le polasetiki, lik'hemik'hale, lijo, meriana, masela le liindasteri tse ling.
Drick o ela hloko temoho ea litalenta le kaho ea sehlopha, ho latela mohopolo oa nts'etsopele oa botsebi, boinehelo.pragmatism, le boqapi.
Ho latela molao-motheo o shebaneng le bareki, rarolla litlhoko tse potlakileng le tse sebetsang tsa bareki, 'me u fane ka litharollo tsa maemo a pele ho bareki ba nang le lihlahisoa tsa boleng bo holimo le theknoloji e tsoetseng pele.