1. Kakaretso

Sheba lipalo tse latelang ha u ntse u bala khaolo ena.

1.1 Selelekela sa Sehlooho

1.1.1 Maemo

TS EN ISO/DIS 22611 Liaparo tsa ts'ireletso khahlano le li-aerosol tse tšoaetsanoang - Mokhoa oa teko oa ho hanyetsa ho kenella ka li-aerosol tse silafalitsoeng ke baeloji.

1.1.2 Litlhaloso

lJenereithara ea aerosol:    Atomizer

lKamore ea ho pepeseha:  PMMA

lKopano ea mohlala :2, tšepe e sa hloekang

lPompo e phofshoana:Ho fihla ho 80kpa

lBoemo: 300mm*300mm*300mm

lPhepelo ea motlakase  :220V 50-60Hz

l Boima ba mochini: 46cm×93cm×49cm (H)

l Boima ba 'mele: 35kg

2. TŠEBELETSO EA SESEBETSI

2.1 Boitokisetso

Kenya likarolo tse tharo ka har'a khabinete ea biosafety. Sheba likarolo tse ling tsa mochine oa teko 'me u netefatse hore likarolo tsohle li sebetsa hantle le ho hokahanya hantle.

Ho khaola lisampole tse robeli joalo ka lisekoere tsa bophara ba 25mm.

Lokisetsa setso sa bosiu ba Staphylococcus aureus ka phetisetso ea aseptic ea baktheria ho tloha ho agar ea limatlafatsi (e bolokiloeng ho 4±1℃) ho ea moro oa limatlafatsi le ho kenngoa ho 37±1℃ holim'a orbital shaker.

Fokotsa setso ka bongata bo loketseng ba letsoai la isotonic le hloekisitsoeng ho fana ka palo ea ho qetela ea baktheria e ka bang 5*10.⁷lisele cm^-3ho sebelisa kamore ea ho bala ea baktheria ea Thoma.

Tlatsa setso se ka holimo ho atomizer. Boemo ba mokelikeli bo pakeng tsa boemo bo holimo le bo tlase.

2.2 Ts'ebetso

Kenya mohlala oa kopano. Beha silicone washer A, lesela la liteko, washer ea silicone B, membrane, ts'ehetso ea terata holim'a sekoaelo se bulehileng, koahela ka setsi.

Kenya mohlala o mong oa kopano ntle le sampole.

Bula sekwahelo se ka holimo sa kamore ea teko.

Kenya mohlala oa kopano ka sampuli le kopano ntle le sampuli ka Fasten ea Fig. 4-1.

Etsa bonnete ba hore li-tubes tsohle li hokahane hantle.

Hokela moea o hatelitsoeng ho lokisa moea o hatelitsoeng.

Sebelisa moea o phallang oa 5L / min ka ho fetola meter ea ho phalla ho atomizer 'me u qale ho hlahisa aerosol.

Ka mor'a metsotso e 3, kenya pompo ea vacumm. E behe 70kpa.

Kamora metsotso e 3, tima moea ho atomizer, empa tlohela pompo ea vacuum e sebetsa bakeng sa 1 min.

Koala pompo ea vaccum.

Tlosa likopano tsa mohlala ho tloha ka kamoreng. 'Me u fetisetse li-membrane tsa 0.45um ka mokhoa o hlakileng libotlolong tsa bokahohle tse nang le 10ml sterile isotonic saline.

Ntša ka ho sisinya bakeng sa 1 min. 'Me u etse li-serial dilutions ka saline e hloekisitsoeng. (10^-1, 10^-2, 10^-3, le 10^-4)

Hlakola li-aliquots tse 1ml tsa dilution e 'ngoe le e 'ngoe ka kopi u sebelisa agar ea limatlafatsi.

Eketsa lipoleiti ka bosiu bo le bong ho 37±1℃ 'me u hlahise liphetho u sebelisa karo-karolelano ea bokamorao ba baktheria ho palo ea baktheria e fetisitsoeng ka sampole ea tlhahlobo.

Etsa liqeto tse 'nè ho mofuta o mong le o mong oa lesela kapa boemo ba lesela.

3. TSAMAISO

Joalo ka lisebelisoa tsohle tsa motlakase, yuniti ena e tlameha ho sebelisoa ka nepo mme tlhokomelo le tlhahlobo li tlameha ho etsoa ka linako tse ling. Mehato e joalo e tla netefatsa ts'ebetso e bolokehileng le e sebetsang ea lisebelisoa.

Tlhokomelo ea nako le nako e kenyelletsa litlhahlobo tse entsoeng ka kotloloho ke moetsi oa liteko le/kapa basebetsi ba lumelletsoeng ba litšebeletso.

Tlhokomelo ea thepa ke boikarabello ba moreki mme e tlameha ho etsoa joalo ka ha ho boletsoe khaolong ena.

Ho hloleha ho etsa liketso tse khothaletsoang tsa tlhokomelo kapa tlhokomelo e etsoang ke batho ba sa lumelloeng ho ka hlakola tiisetso.

1. Mochini o tlameha ho hlahlojoa ho thibela ho lutla ha likhokahano pele ho liteko;

2. Ho tsamaisa mochine ho thibetsoe ha u o sebelisa;

3. Khetha motlakase o lumellanang le motlakase le motlakase. U se ke ua phahameng haholo ho qoba tukang sesebelisoa;

4. Ka kopo ikopanye le rona e le hore u sebetsane le nako ha mochine o sa sebetse;

5. E tlameha ho ba le tikoloho e ntle ea moea ha mochine o sebetsa;

6. Ho hloekisa mochine ka mor'a tlhahlobo nako le nako;