Gutondekanya no gushyira mu bikorwa Kjeldahl Isesengura rya Azote

I. Itondekanya rya Azote Igikoresho

Igikoresho cyo Kugena Azote ni ubwoko bwibikoresho byubushakashatsi bikoreshwa mukumenya ibirimo azote mu bintu, bikoreshwa cyane mubice byinshi nka chimie, ibinyabuzima, ubuhinzi, ibiryo nibindi. Ukurikije amahame atandukanye yakazi hamwe nuburyo bukoreshwa, Igikoresho cyo Kugena Azote gishobora kugabanywamo ubwoko butandukanye.

1. Kjeldahl Igikoresho cyo Kugena Azote:

Kjeldahl Igikoresho cyo Kugena Azote nuburyo bwa kera bwo kugena azote, bushingiye ku ihame rya reaction ya Kjeldahl (uburyo bwa Kjeldahl). Iharura ibirimo azote mu cyitegererezo ihindura azote kama muri sample ikabamo azote ya ammoniacal, hanyuma ikinjiza ammonia hamwe na aside ikora umunyu wa amonium, hanyuma ikagena umunyu wa amonium ukoresheje titre ya aside isanzwe. Igikoresho cya Kjeldahl Igikoresho cya Azote gifite ibiranga imikorere yoroshye, ibisubizo nyabyo kandi byizewe, ariko isesengura ryigihe kirekire, kandi inzira yo gukoresha aside sulfurike, alkali ikomeye nizindi reagent ziroroshye gutera umwanda kubidukikije.

2. Igikoresho cyo Kugena Azote:

Igikoresho cya Dumas Azote ikoresha uburyo bwo gutwika ubushyuhe bwo hejuru (uburyo bwa Dumas) kugirango hamenyekane ibirimo azote muri sample. Icyitegererezo gitwikwa ku bushyuhe bwinshi mu bidukikije bya ogisijeni, aho azote kama ihindurwamo azote, hanyuma ibirimo azote bikamenyekana na gaz chromatografiya hamwe nubundi buhanga, kugirango babare azote iri muri sample. Dumas Nitrogen Kumenya byihuse mubisesengura kandi byangiza ibidukikije kuko bidasaba gukoresha reagent zifite ubumara kandi bwangiza. Nyamara, igiciro cyibikoresho ni kinini, kandi ibisabwa kugirango umuntu yitegure ni byinshi.

3. Ultraviolet spectrophotometric nitrogen isesengura:

UV isesengura rya azote isesengura ishingiye kuri tekinoroji ya ultraviolet isesengura rya azote. Azote iri muri sample ikora hamwe na reagent yihariye kugirango itange ibara ryamabara, kandi ibirimo azote murugero birashobora kubarwa mugupima ultraviolet iyinjizwa ryikigo. Ubu bwoko bwa azote yisesengura biroroshye gukora kandi byihuse kubisesengura, ariko birashobora kubangikanywa nibindi bintu murugero, bikagira ingaruka kubisubizo byukuri.
4. Igikoresho cyo Kugena Azote Yikora:
Deteter ya azote ikomatanya ihuza ibyiza byubuhanga butandukanye bwo kugena azote kugirango igere kuri azote ikora kandi ifite ubwenge. Binyuze mu kugenzura mudasobwa, ihita yuzuza intambwe zo gupima icyitegererezo, kongeramo icyitegererezo, reaction no gutahura, bitezimbere cyane gusesengura neza. Muri icyo gihe, isesengura rya azote ryikora naryo rifite imirimo yo kubika amakuru, raporo yo gucapa, nibindi, byorohereza abakoresha gukora gucunga amakuru no gusesengura ibisubizo.

II. Gushyira mu bikorwa Igikoresho cyo Kugena Azote

Deteter ya azote ifite intera nini ya porogaramu mubice byinshi, ibikurikira nibyo byingenzi bikoreshwa:

1. Kwipimisha umutekano wibiribwa: Igikoresho cyo Kugena Azote kirashobora gukoreshwa muguhitamo proteine ​​mubiribwa. Binyuze mu kugena ibirimo azote mu biribwa, ibirimo poroteyine birashobora kubarwa mu buryo butaziguye, bitanga ibisobanuro byingenzi byo gupima umutekano w’ibiribwa. Byongeye kandi, isesengura rya azote rishobora kandi gukoreshwa mu kumenya inyongeramusaruro mu biribwa, ibisigisigi byica udukoko n’ibindi bintu byangiza, kugira ngo umutekano w’ibiribwa.

2. Ubushakashatsi mu buhinzi: Mu bushakashatsi bw’ubuhinzi, metero ya azote irashobora gukoreshwa kugirango hamenyekane ibinyabuzima bya azote mu butaka no mu ngingo z’ibimera. Mugusobanukirwa nimirire ya azote yubutaka nibimera, irashobora gutanga ubumenyi bwa siyanse yo gufumbira ibihingwa no guteza imbere imikurire niterambere ryibihingwa.

3. umusaruro wimiti: mugikorwa cyo gukora imiti, metero ya azote irashobora gukoreshwa kugirango hamenyekane azote yibikoresho fatizo nibicuruzwa. Binyuze mu gihe gikwiye cyo kugenzura ihinduka rya azote mugikorwa cyumusaruro, ibipimo byumusaruro birashobora guhinduka mugihe gikwiye kugirango ubuziranenge bwibicuruzwa nibikorwa neza.

4. Gukurikirana ibidukikije: isesengura rya azote rishobora gukoreshwa mu bwiza bw’amazi, ikirere n’izindi ngero z’ibidukikije mu kugena ibirimo azote. Mugusobanukirwa ihinduka rya azote mu ngero z’ibidukikije, irashobora gusuzuma uko ihumana ry’ibidukikije ryifashe kandi igatanga inkunga y’amakuru yo gukurikirana ibidukikije n’imiyoborere.

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