Hoʻopā i nā ʻāpana mea hana PCR nui Fluorescence
ʻO ka wehewehe pōkole:
Helu kumu hoʻohālike: CFX96 Touch 1. ʻO ke kaiapuni hana 1.1 Hana wela 5-31 ℃ 1.2 Haʻahaʻa hana ʻO ka haʻahaʻa haʻahaʻa pili ≤80% 1.3 Ka mana hana 100-240 VAC, 50-60Hz. 2. Hana Hiki ke hoʻohana ʻia i ka helu nucleic acid, ka nānā ʻana i ka pae hōʻike gene, ka ʻike mutation gene, ka ʻike GMO a me ka ʻike kikoʻī huahana a me nā kahua noiʻi ʻē aʻe. 3. Nā koi hana a me nā ʻenehana 3.1 Hana Nui (* ʻo ia ka hōʻailona e pono e hoʻokō ʻia) *3.1.1 ʻEono ʻike channe...
Helu kumu hoʻohālike: CFX96 Touch
1. Ka nohona hana
1.1 Ka wela hana 5-31 ℃
1.2 ʻO ka haʻahaʻa hana ʻO ka haʻahaʻa pili ≤80%
1.3 Ka lako mana hana 100-240 VAC, 50-60Hz.
2. Function
Hiki ke hoʻohana ʻia i ka helu nucleic acid, ka nānā ʻana i ka pae hōʻike gene, ka ʻike mutation gene,
ʻO ka ʻike GMO a me ka nānā ʻana i nā huahana kikoʻī a me nā kahua noiʻi ʻē aʻe.
3. Nā koi hana a me nā ʻenehana
3.1 Hana Nui (* ʻo ia ka hōʻailona e pono e hoʻokō ʻia)
*3.1.1 ʻEono mau ala ʻike, hiki ke hoʻomaopopo i ka PCR 5-fold, hiki ke ʻike i nā ʻano genes 5 i ka manawa like, a me ke kahawai ʻike FRET kūikawā.
*3.1.2 Me ka hana PCR gradient mahana wela, hiki ke holo i 8 mau mahana like ole i ka manawa like, a ua like ka manawa incubation o kela me keia wela.
3.1.3 Reagent hāmama loa, kūpono no nā ʻano noiʻi ʻepekema a me nā reagents lāʻau lapaʻau
3.1.4 He kūpono no nā ʻano fluorescence like ʻole, e like me Taqman, Molecular Beacon, FRET probe, SYBR Green I, etc.
3.1.5 Ua wehe ʻia nā mea hoʻohana a hiki ke hoʻohana i ka paipu hoʻokahi 0.2ml, ʻewalu paipu, pā 96-well, etc.
*3.1.6 Hiki ke holo kūʻokoʻa a hana ma waho. Hiki ke nānā ʻia ka pihi hoʻonui PCR fluorescence manawa maoli me ka ʻole o ka pili kamepiula
3.2 Nā koi ʻenehana nui (* ʻo ia ka hōʻailona e pono e hoʻokō ʻia)
*3.2.1 Laʻana mana: 96×0.2ml, hiki ke hoʻohana maʻamau kikoʻī 96-well pā (12×8)
Ke ʻano o nā mea hoʻopau: 0.2ml paipu hoʻokahi, ʻewalu paipu, 96-well plate, etc
3.2.3 Pūnaehana pane: 1-50µ L (10-25µ L manaʻo ʻia)
*3.2.4 Puna māmā: ʻeono mau alakaʻi me nā kānana
*3.2.5 ʻIke: ʻeono diodes photosensitive me nā kānana
*3.2.6 ka emi ana o ka wela: 5℃/s
3.2.7 Manaʻo hoʻomalu wela: 0-100 ℃
Pono ka wela: ±0.2 ℃ (ma 90˚C)
Kaulike mahana: ± 0.4 ℃ (a hiki i 90˚C i loko o 10 kekona)
*3.2.10 Hana gradient mahana wela: holo 8 mau wela like ʻole i ka manawa like; Ka hoʻomalu ʻana o ka mahana wela: 30-100 ℃; ʻO ke ana wela laulima: 1-24 ℃; Ka manawa incubation ma ka wela gradient: like
3.2.11 Hōʻeuʻeu / hoʻokuʻu hawewe lōʻihi: 450-730nm
3.2.12 ʻIke: Hiki iā ia ke ʻike i nā genes kope hoʻokahi i loko o ka genome kanaka
3.2.13 Ka laulā ikaika: 10 kauoha o ka nui
3.2.14 Hōʻike: 8.5-ʻīniha kala hoʻopā pale
3.2.15 Keʻanoʻikepiliʻikepili: quantitative standard curve, fusion curve, CT or δ δ CT gene expression analysis, multiple internal reference gene analysis and amplification efficiency calculation, gene expression analysis of multiple data files, allele analysis, end point analysis, with alleles , fusion curve analysis
3.2.16 Hoʻouna ʻikepili: Excel, Word, a i ʻole PowerPoint. Loaʻa i nā hōʻike mea hoʻohana nā ʻōkuhi holo, nā kiʻi a me nā hopena ʻikepili i hiki ke paʻi pololei ʻia a mālama ʻia ma ke ʻano he PDF.
*3.2.17 Haʻawina hoʻolālā Chromosome: Ua hoʻohana ʻia ke ʻano PCR manawa maoli no ka hoʻohālikelike ʻana i ke ʻano o ka chromatin ma o ka hoʻohālikelike ʻana i nā hopena o nā nucleases i ka hōʻino ʻana o DNA genomic. Hōʻike maoli kēia i ka pilina kiʻekiʻe ma waena o ka hoʻolālā chromatin a me ka hōʻike ʻana o ke ʻano
4 Mea Pono
ʻO ka polokalamu kamepiula a me ka nānā ʻana (me ka helu piha, ka helu pili, ka hoʻoheheʻe ʻana i ka curve, ka nānā ʻana i ka hopena, ka hoʻohālikelike ʻikepili multi-board, etc.)
5 Manawa hoʻopaʻa maikaʻi
ʻO ka manawa hōʻoia maikaʻi ʻo hoʻokahi makahiki ma hope o ka hoʻokomo ʻana a me ka ʻae ʻia ʻana o ke kauoha e ka mea hoʻohana.
SHANDONG DRICK INSTRUMENTS CO.,LTD
ʻIkepili Hui
ʻO Shandong Drick Instruments Co., Ltd, ke hana nui nei i ka noiʻi a me ka hoʻomohala ʻana, ka hana ʻana a me ke kūʻai ʻana i nā mea hoʻāʻo.
Ua hoʻokumu ʻia ka hui ma 2004.
Hoʻohana ʻia nā huahana i nā ʻāpana noiʻi ʻepekema, nā keʻena nānā ʻana i ka maikaʻi, nā kulanui, ka ʻeke, ka pepa, ka paʻi ʻana, ka lāʻau a me nā plastics, nā kemika, nā meaʻai, nā lāʻau lapaʻau, textiles, a me nā ʻoihana ʻē aʻe.
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ʻO ka mālama ʻana i ke kumu kūʻai aku, hoʻoponopono i nā pono wikiwiki a kūpono o nā mea kūʻai aku, a hāʻawi i nā hāʻina papa mua i nā mea kūʻai aku me nā huahana kiʻekiʻe a me nā ʻenehana holomua.
